Journal: Cell reports

Article Title: Nanoparticle-based itaconate treatment recapitulates low-cholesterol/low-fat diet-induced atherosclerotic plaque resolution

doi: 10.1016/j.celrep.2024.114911

Figure Lengend Snippet: (A) Relative levels of the metabolites of the TCA cycle in whole aortas from CPC. White/black square on bottom left denotes not detected. (B) SIRM analysis of itaconate isotopologs in CPC aortas from (A). Bottom: scheme illustrating the production of itaconate. Data are presented as mean ± SD. p values were obtained from Student’s t-test. (C) qPCR analysis of the levels of Irg1 transcripts in whole aortas from separate CPC cohorts. N.d., not detected. Data are presented as mean ± SD. p values were obtained from Student’s t-test. (D) Immunofluorescence micrographs depicting positive staining for IRG1 and CD68 in BCA lesions from Prog mice with DAPI counterstain. Scale bars, 100 and 20 μm. (E and F) Histologic assessment with H&E and Movat pentachrome stains and immunofluorescence analysis of IRG1 and CD68 expression in human (F) stable vs. (E) vulnerable thin-cap fibroatheroma (TCFA) plaques from mid-left anterior descending artery (MLAD) with DAPI counterstain. Quantification of co-localization between IRG1 and CD68 is included in (F). Scale bars, 1 mm and 100 μm. (G) BMDMs were treated with free cholesterol for 48 h in the presence or absence of acyl-coenzyme A cholesterol acyltransferase inhibitor (ACAT-i) followed by qPCR mRNA analysis of the expression of Irg1 and Il6 as indicated. Data are presented as mean ± SD. p values were obtained from ANOVA with Tukey post hoc. (H) Immunoblot of IRG1 knockdown in iBMDMs using shRNA. (I) iBMDMs with the IRG1 knockdown (IRG1 KD) or control cells were treated with cholesterol as in (G), and the levels of IkBα and iNOS were determined in the cell lysates using immunoblotting. Staining with Coomassie brilliant blue (CBB) was used as total protein loading control.

Article Snippet: anti-human CD68 (KP1) mouse mAb , ThermoFisher Scientific , Cat#: 14-0688-82; RRID:AB_11151139.

Techniques: Immunofluorescence, Staining, Expressing, Western Blot, Knockdown, shRNA, Control

Journal: Cell reports

Article Title: Nanoparticle-based itaconate treatment recapitulates low-cholesterol/low-fat diet-induced atherosclerotic plaque resolution

doi: 10.1016/j.celrep.2024.114911

Figure Lengend Snippet:

Article Snippet: anti-human CD68 (KP1) mouse mAb , ThermoFisher Scientific , Cat#: 14-0688-82; RRID:AB_11151139.

Techniques: Purification, Plasmid Preparation, Produced, Recombinant, Concentration Assay, Saline, Labeling, Membrane, Enzyme-linked Immunosorbent Assay, Quantitation Assay, Phospholipid Assay, BIA-KA, RNAscope, HD Assay, Polymer, Sequencing, Gene Expression, Software, Microscopy

Journal: Neural Regeneration Research

Article Title: Surgical intervention combined with weight-bearing walking training promotes recovery in patients with chronic spinal cord injury: a randomized controlled study

doi: 10.4103/NRR.NRR-D-23-01198

Figure Lengend Snippet: Histopathological changes in the spinal cord of a patient who underwent surgical detethering 9 months after injury. Low (A, C) and high (B, D) magnification images. B and D represent the boxes in A and C, respectively. KP1-positive activated microglia and macrophages are visualized. Some cells exhibit amoeboid morphology. Cytoplasm filled with phagocytic vesicles is indicated by solid arrows. Activated microglia/macrophages clumped together are indicated by hollow arrows. Scale bars: 200 μm in A, C; 50 μm in B, D.

Article Snippet: Paraformaldehye-fixed paraffin-embedded spinal cord tissue samples were shipped to Shanghai Jiao Tong University School of Medicine, where sections (4 μm) were stained with mouse anti-human CD68 (KP1, Dako, Glostrup, Denmark).

Techniques:

Journal: iScience

Article Title: β-chitosan attenuates hepatic macrophage-driven inflammation and reverses aging-related cognitive impairment

doi: 10.1016/j.isci.2024.110766

Figure Lengend Snippet:

Article Snippet: Mouse monoclonal anti-CD68 , Novus Biologicals , Cat#NB100-683.

Techniques: Plasmid Preparation, Recombinant, Lysis, Membrane, Staining, Enzyme-linked Immunosorbent Assay, Bicinchoninic Acid Protein Assay, Western Blot, Software, Expressing, Gene Expression

Journal: Neurology® Neuroimmunology & Neuroinflammation

Article Title: Single-Cell Transcriptomic Analyses of Brain Parenchyma in Patients With New-Onset Refractory Status Epilepticus (NORSE)

doi: 10.1212/NXI.0000000000200259

Figure Lengend Snippet: Iba-1 revealed gliosis for patients with TLE or NORSE. CD68 highlighted the infiltration of macrophages in the parenchyma of NORSE#1. CD163 revealed perivascular macrophages for TLE#2 and TLE#4. Scale bars: 50 µm.

Article Snippet: Three micrometers–thick tissue sections were stained by hematoxylin-eosin or were processed for immunostaining by a fully automated stained Ultra (Ventura, Roche) using diaminobenzidine as a chromogen and the following primary antibodies and dilutions: monoclonal mouse clone MRQ-26 anti-CD163 (Roche, prediluted), monoclonal mouse clone KP1 anti-CD68 (Agilent technologies, 1/1,000), and polyclonal rabbit anti-Iba1 (Wako, 1/500).

Techniques:

Journal: iScience

Article Title: Defects of renal tubular homeostasis and cystogenesis in the Pkhd1 knockout

doi: 10.1016/j.isci.2024.109487

Figure Lengend Snippet:

Article Snippet: Mouse monoclonal, Anti-CD68 (KP1) , Thermo Fisher Scientific , Cat# 14-0688-82 RRID: 11151139.

Techniques: Recombinant, Knock-Out, Blocking Assay, Protease Inhibitor, Bicinchoninic Acid Protein Assay, SYBR Green Assay, Reverse Transcription, In Situ, Software, Microscopy, Imaging